Vimentin antibody (C-Term)

Catalog No. ABIN7538944

This Mouse Monoclonal antibody specifically detects Vimentin in IHC, IHC (p) and HIER. It exhibits reactivity toward Human.

Quick Overview for Vimentin antibody (C-Term) (ABIN7538944)

Target: Vimentin (VIM)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Clone: SRL33

Product Details anti-Vimentin Antibody

Binding Specificity: C-Term

Purpose: Liquid Mouse Monoclonal Antibody Vimentin

Specificity: Human vimentin.

Characteristics: This product is intended for the qualitative identification by light microscopy of Vimentin molecules in paraffin sections.

Purification: Tissue culture supernatant

Immunogen: Prokaryotic recombinant protein corresponding to a portion of the C-terminus of the human vimentin molecule.

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Suggested dilution: 1:400 for 30 minutes at 25°C. Heat induced epitope retrieval using Epitope Retrieval Solution pH 9.0. This is provided as a guide and users should determine their own optimal working dilutions.,Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 60 mg/L

Buffer: This product is a liquid tissue culture supernatant containing 15 mM sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Pathways: Caspase Cascade in Apoptosis