His Tag antibody

Catalog No. ABIN7539828

This Mouse Monoclonal antibody specifically detects His Tag in WB, ELISA, IP, IHC, ICC and FACS. It exhibits reactivity toward Tag.

Quick Overview for His Tag antibody (ABIN7539828)

Target: His Tag

Reactivity: Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 6A8H8

Product Details anti-His Tag Antibody

Purpose: His Tag Antibody

Purification: Purified antibody

Immunogen: Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/100 - 1/1000

Restrictions: For Research Use only

Handling

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for His Tag

Target: His Tag

Target Type: Tag

Background: The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.