Endoglin antibody (APC)

Catalog No. ABIN7540413

The Mouse Monoclonal anti-Endoglin antibody has been validated for FACS. It is suitable to detect Endoglin in samples from Human and Pig.

Quick Overview for Endoglin antibody (APC) (ABIN7540413)

Target: Endoglin (ENG)

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Endoglin antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: MEM-229

Product Details anti-Endoglin Antibody (APC)

Purpose: Anti-Hu CD105 APC

Specificity: The antibody MEM-229 recognizes an extracellular epitope of CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow, it is also present on syncytiotrophoblast on placenta throughout pregnancy.

No Cross-Reactivity: Dog, Horse

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: CD105

Background:

NCBI Full Gene Name: endoglin

Description: CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

Other names: Endoglin, END, ENG, HHT1, ORW1

Gene name: ENG

Gene ID: 2022

UniProt: P17813