NGFR antibody (FITC)

Catalog No. ABIN7540431

The Mouse Monoclonal anti-NGFR antibody is suitable to detect NGFR in samples from Human, Non-Human Primate, Rabbit, Cat and Ferret. It has been validated for FACS and ICFC.

Quick Overview for NGFR antibody (FITC) (ABIN7540431)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Human, Non-Human Primate, Rabbit, Cat, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)

Clone: NGFR5

Product Details anti-NGFR Antibody (FITC)

Purpose: Anti-Hu CD271 FITC

No Cross-Reactivity: Mouse, Rat

Characteristics: The mouse monoclonal antibody NGFR5 (originally C34C) recognizes an epitope within ammino acids 1 - 160 of CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily.

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Purified CD271 protein isolated from human melanoma cell line A875

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Extracellular and intracellular staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze,Protect from prolonged exposure to light

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: CD271

Background:

NCBI Full Gene Name: nerve growth factor receptor

Description: CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.

Other names: TNFRSF16, NGFR, p75NTR, p75NGFR

Gene name: NGFR

Gene ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding