EEF1A2 antibody (C-Term)

Catalog No. ABIN7540568

Supplier Product No.: 600-101-ml6s

This Goat Polyclonal antibody specifically detects EEF1A2 in WB, ELISA, IHC, IF and FM. It exhibits reactivity toward Human.

Quick Overview for EEF1A2 antibody (C-Term) (ABIN7540568)

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EEF1A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-EEF1A2 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-101-ml6s

Supplier: Rockland

Purpose: EEF1A2 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human EEF1A2.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-EEF1A2 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal portion of human EEF1A2 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:200

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-EEF1A2 Antibody has been tested in WB, IF, and IHC.  Expect a band at ~50.5 kDa in western blot using appropriate lysates.  Positive control used: human skeletal muscle lysate, MCF-7 nuclear extract lysate in WB, MCF-7 cells in IF, and human skeletal muscle tissue in IHC.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for EEF1A2

Target: EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Alternative Name: EEF1A2

Background: Goat Anti-EEF1A2 Antibody, Goat Anti-Eukaryotic Translation Elongation Factor 1 Alpha 2 Antibody, Eukaryotic Elongation Factor 1 A-2, EF-1-Alpha-2, Statin-S1, EEF1AL, STN 3, Elongation Factor 1-Alpha 2, Statin-Like, EEF1A-2, EIEE33, Statin, MRD38, EF1A, STNL, HS1,EEF1A2 (Eukaryotic Translation Elongation Factor 1 Alpha 2) protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. EEF1A2 may be associated with the development of ovarian cancer, along with Epileptic Encephalopathy, Early Infantile, 33 and Mental Retardation, Autosomal Dominant 38. Anti-EEF1A2 Antibody is useful for researchers interested in cancer research, translation factors, GTP binding, and GTPase activities.

Gene ID: 1917

NCBI Accession: NP_001949

UniProt: Q05639