MAPK14 antibody (pThr180, pTyr182)

Catalog No. ABIN7540583

Supplier Product No.: 600-401-c90

This Rabbit Polyclonal antibody specifically detects MAPK14 in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for MAPK14 antibody (pThr180, pTyr182) (ABIN7540583)

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MAPK14 Antibody

Binding Specificity: pThr180, pTyr182

Supplier Product No.: 600-401-c90

Supplier: Rockland

Purpose: p38 MAPK phospho T180/phospho Y182 Antibody

Cross-Reactivity (Details): antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Purification: Anti-p38 MAPK pT180/pY182 Antibody is directed against p38 MAPK phosphorylated at T180 and Y182.

Immunogen: Anti-p38 MAPK pT180/pY182 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:250

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-p38 MAPK pT180/pY182 Antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 39kDa in size corresponding to p38 MAPK protein phosphorylated at Thr180/Tyr182 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Alternative Name: Mapk14

Background: Mitogen-activated protein kinase 14, MAP kinase 14, MAPK 14, CRK1, Mitogen-activated protein kinase p38 alpha, MAP kinase p38 alpha,p38 MAPK pT180/pY182 Antibody detects phosphorylated p38 MAPK protein. The three Mitogen-Activated Protein Kinases (MAPKs) are evolutionarily conserved protein kinases that control a vast array of cellular processes. p38 MAPK is one of these kinases and it is activated by both inflammatory cytokines and by stress. The p38 MAPK is thought to be particularly important in diseases like asthma and autoimmunity but it also plays important roles in the stress response of the nervous system. Like the other MAPKs, p38 is activated by a dual specificity kinase that phosphorylates Thr180 and Tyr182. Anti-p38 MAPK pT180/pY182 Antibody is ideal for investigators involved in Signal Transduction and Apoptosis research.

Gene ID: 70496

NCBI Accession: NM_031020

UniProt: P70618

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins