GRIN1/NMDAR1 antibody

Catalog No. ABIN7540585

Supplier Product No.: 600-401-d85

This anti-GRIN1/NMDAR1 antibody is a Rabbit Polyclonal antibody detecting GRIN1/NMDAR1 in WB and IHC. Suitable for Rat.

Quick Overview for GRIN1/NMDAR1 antibody (ABIN7540585)

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN1/NMDAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GRIN1/NMDAR1 Antibody

Supplier Product No.: 600-401-d85

Supplier: Rockland

Purpose: NMDA R1, Splice Variant C1 Antibody

Cross-Reactivity (Details): Anti-NMDA R1, Splice Variant C1 antibody is directed against NMDA R1 receptor protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-NMDA R1, Splice Variant C1 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, C1 splice variant insert.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:1000-2000

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-NMDA R1, Splice Variant C1 (Rabbit) Antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 120 kDa in size corresponding to the NR1 subunit of the NMDA receptor containing the C1 splice variant insert in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 50μL

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for GRIN1/NMDAR1

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Alternative Name: Grin1

Background: Glutamate [NMDA] receptor subunit zeta-1,N-methyl-D-aspartate receptor subunit NR1, NMD-R1, Grin1,NMDA R1, Splice Variant C1 antibody detects NMDA R1receptor protein. The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory and neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death. There are a number of different splice variants of the NR1-Subunit. Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR. Anti-NMDA R1, Splice Variant C1 Antibody is ideal for investigators involved in Neuroscience and Signal Transduction research.

Gene ID: 24408

NCBI Accession: NP_058706

UniProt: P35439

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity