SQSTM1 antibody (Internal Region)

Catalog No. ABIN7540594

Supplier Product No.: 600-401-hb8s

This anti-SQSTM1 antibody is a Rabbit Polyclonal antibody detecting SQSTM1 in WB, IHC, ELISA, IF and FM. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for SQSTM1 antibody (Internal Region) (ABIN7540594)

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SQSTM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-SQSTM1 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-hb8s

Supplier: Rockland

Purpose: SQSTM1/p62 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human SQSTM1/p62.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-SQSTM1/p62 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human SQSTM1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:200

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-SQSTM1/p62 Antibody has been tested in ELISA, WB, IF, and IHC.  Expect a band at ~38, 47kDa in western blot using appropriate tissues and lysates.  Positive control used: U87-MG, HeLa, and Mouse liver lysates in western blot, Human skeletal muscle in Immunohistochemistry, and HeLa in immunofluorescence.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-SQSTM1 antibody (ABIN7540594)

Dalle Carbonare, Minoia, Vareschi, Piritore, Zouari, Gandini, Meneghel, Elia, Lorenzi, Antoniazzi, Pessoa, Zipeto, Romanelli, Guardavaccaro, Valenti: "Exploring the Interplay of RUNX2 and CXCR4 in Melanoma Progression." in: Cells, Vol. 13, Issue 5, (2024) (PubMed).

Target Details for SQSTM1

Target: SQSTM1 (Sequestosome 1 (SQSTM1))

Alternative Name: SQSTM1

Background: Rabbit Anti-Sequestosome 1 Antibody, Phosphotyrosine-Independent Ligand For The Lck SH2 Domain Of 62 KDa, EBI3-Associated Protein Of 60 KDa, Ubiquitin-Binding Protein P62, Oxidative Stress Induced Like, EBIAP, OSIL, P60, Phosphotyrosine Independent Ligand For The Lck SH2 Domain P62, EBI3-Associated Protein P60, Paget Disease Of Bone, Sequestosome-1, FTDALS3, NADGP, A170, DMRV, ZIP3, P62B, PDB3, ORCA, P62,SQSTM1(Sequestosome 1)/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It functions as a bridge between polyubiquitinated cargo and autophagosomes and interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family.  Along with WDFY3, SQSTM1 is involved in the formation and autophagic degradation of cytoplasmic ubiquitin-containing inclusions (p62 bodies, ALIS/aggresome-like induced structures) and is required to recruit ubiquitinated proteins to PML bodies in the nucleus. It may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1 and may play a role in titin/TTN downstream signaling in muscle cells. It is an adapter that mediates the interaction between TRAF6 and CYLD.  SQSTM1 may be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. It is involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Mutations in this gene result in frontotemporal dementia and/or amyotrophic lateral sclerosis, and familial Paget disease of bone.  Anti-SQSTM1 Antibody is useful for researchers interested in Cancer Research, Neuroscience, and Cytokines & Growth Factor Antibodies. 

Gene ID: 8878

NCBI Accession: NP_001135770

UniProt: Q13501

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy