Connexin 43/GJA1 antibody (Internal Region)

Catalog No. ABIN7540609

Supplier Product No.: 600-401-mj2

This anti-Connexin 43/GJA1 antibody is a Rabbit Polyclonal antibody detecting Connexin 43/GJA1 in WB, IHC, ELISA, IF and FM. Suitable for Mouse.

Quick Overview for Connexin 43/GJA1 antibody (Internal Region) (ABIN7540609)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-mj2

Supplier: Rockland

Purpose: Connexin 43 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against rat connexin 43.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Connexin 43 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse connexin 43 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:15,000

Immunohistochemistry_Dilution: 1:200

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-Connexin 43 Antibody has been tested in ELISA, WB, IF, and IHC. Expect a band at ~43Da in western blot using appropriate lysates. Positive control used: Mouse Heart in IHC, Rat Pup Brain cells, NIH-3T3, or HEK293T in IF, and HEK293T, Mouse Heart, or Rat Brain in WB.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: Gja1

Background: Rabbit Anti-Connexin 43 Antibody, Rabbit Anti-Gja1 Antibody, Gap Junction Protein Alpha 1, Gap Junction Protein, Alpha 1, 43 kDa, Gap Junction 43 KDa Heart Protein, Connexin-43, GJAL, Gap Junction Protein, Alpha 1, 43 kDa (Connexin 43), Oculodentodigital Dysplasia (Syndactyly Type III), Gap Junction Protein, Alpha-Like, Gap Junction Alpha-1 Protein, Connexin 43, AVSD3, EKVP3, HLHS1, PPKCA, CMDR, CX43, EKVP, ODDD, Cx43, HSS,Connexin 43 or GJA1 (Gap Junction Protein Alpha 1) is a gap junction protein that acts as a regulator of bladder capacity. Gap channels (gap junctions) are specialized cell-cell contacts that provide direct intracellular communication. They allow passive diffusion of molecules up to 1 kDa, including nutrients, metabolites (glucose), ions (K+, Ca2+) and second messengers (IP3, cAMP). It may play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. Connexin 43 may play a role in cell growth inhibition through the regulation of NOV expression and localization. It plays an essential role in gap junction communication in the ventricles. Anti-Connexin 43 Antibody is useful for researchers interested in EGF/EGFR Signaling Pathways or signaling receptor binding.

Gene ID: 14609

NCBI Accession: NP_034418

UniProt: P23242

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization