MMP2 antibody (Internal Region)

Catalog No. ABIN7540614

Supplier Product No.: 600-401-mj9s

The Rabbit Polyclonal anti-MMP2 antibody has been validated for WB, IHC, ELISA, IF and FM. It is suitable to detect MMP2 in samples from Human.

Quick Overview for MMP2 antibody (Internal Region) (ABIN7540614)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-MMP2 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-mj9s

Supplier: Rockland

Purpose: MMP2 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human MMP2.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-MMP2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human MMP2 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-MMP2 Antibody has been tested in ELISA, WB, IF, and IHC.  Expect a band at ~ 65, 74kDa in western blot using appropriate lysates.  Positive control used: Human placenta and Human lung lysates, as well as HEK293T MMP2 overexpressed lysate in WB, HepG2 and U87MG cells for IF, Human metastatic breast carcinoma in lymph nodes for IHC.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Alternative Name: MMP2

Background: Rabbit Anti-MMP2 Antibody, Rabbit Anti-Matrix Metallopeptidase 2 Antibody, Matrix Metalloproteinase-2, CLG4A, MMP-2, TBE-1, Matrix Metalloproteinase 2 Gelatinase A 72 kDa Type IV Collagenase), Matrix Metallopeptidase 2 Gelatinase A 72 kDa Type IV Collagenase), Matrix Metalloproteinase-II, 72 KDa Type IV Collagenase, Collagenase Type IV-A, Neutrophil Gelatinase, 72 KDa Gelatinase, Gelatinase A, EC 3.4.24.24, MMP-II, MONA, CLG4,Matrix metalloproteases (matrix metalloproteinase, MMPs), also called matrixins, are zinc-dependent endopeptidases and the major proteases in ECM degradation. MMPs can degrade several extracellular molecules and several bioactive molecules. MMP2 is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellularly by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, metastasis, tissue repair, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, it can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. MMP2 also cleaves KISS at a Gly- -Leu bond. It appears to have a role in myocardial cell death pathways and contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro and is involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. MMP2 is a ligand for integrin/beta3 on the surface of blood vessels. Anti-MMP2 antibody is useful for researchers interested in osteolysis, nodulosis, arthropathy, Apoptosis Research, and Cytokines & Growth Factor Antibodies.

Gene ID: 4313

NCBI Accession: NP_001121363

UniProt: P08253

Pathways: Activation of Innate immune Response