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p130 antibody (C-Term)

This Rabbit Polyclonal antibody specifically detects p130 in WB and ELISA. It exhibits reactivity toward Human.
Rockland
Catalog No. ABIN7540634
Supplier Product No.: 600-401-mp1

Quick Overview for p130 antibody (C-Term) (ABIN7540634)

Target

See all p130 (RBL2) Antibodies
p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Reactivity

  • 66
  • 26
  • 26
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
Human

Host

  • 62
  • 5
Rabbit

Clonality

  • 60
  • 7
Polyclonal

Conjugate

  • 31
  • 5
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This p130 antibody is un-conjugated

Application

  • 27
  • 23
  • 13
  • 13
  • 10
  • 7
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  • 5
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA
  • Binding Specificity

    • 15
    • 8
    • 7
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    • 6
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    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    Supplier Product No.

    600-401-mp1

    Supplier

    Rockland

    Purpose

    p130 Antibody

    Cross-Reactivity (Details)

    This affinity purified antibody is directed against human Retinoblastoma-like protein 2.

    Purification

    The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

    Sterility

    Sterile filtered

    Immunogen

    Anti-p130 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal portion of human Retinoblastoma-like protein 2 conjugated to Keyhole Limpet Hemocyanin (KLH).

    Isotype

    IgG
  • Application Notes

    ELISA_Dilution: 1:10,000 - 1:50,000

    Western_Blot_Dilution: 1 μg/mL

    Comment

    Anti-p130 Antibody has been tested in ELISA and WB.  Expect bands ~128.4 kDa in western blot using appropriate tissues or lysates.  Positive control used: MOLT-4 lysate in western blot.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Expiry Date

    12 months
  • Target

    p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

    Alternative Name

    RBL2

    Background

    Rabbit Anti-p130 Antibody, Rabbit Anti-Retinoblastoma-like protein 2 Antibody, RB Transcriptional Corepressor Like 2, 130 KDa Retinoblastoma-Associated Protein, Retinoblastoma-Related Protein 2, Retinoblastoma-Like Protein 2, Retinoblastoma-Like 2, RBR-2,Retinoblastoma-Associated Protein (130 KDa) is a key regulator of entry into cell division. p130 is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression and controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters and is a potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. It binds to cyclins A and E, as well as, binds to and may be involved in the transforming capacity of the adenovirus E1A protein. p130 may act as a tumor suppressor. Anti-p130 Antibody may be useful for researchers interested in Adipogenesis, Retinoblastoma, and Retinal Cancer.

    Gene ID

    5934

    NCBI Accession

    NP_001310537

    UniProt

    Q08999

    Pathways

    Cell Division Cycle, Mitotic G1-G1/S Phases
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