Tyrosine Hydroxylase antibody (pSer40)

Catalog No. ABIN7540679

Supplier Product No.: 600-401-c99

This Rabbit Polyclonal antibody specifically detects Tyrosine Hydroxylase in WB, IHC, ELISA and FM. It exhibits reactivity toward Rat.

Quick Overview for Tyrosine Hydroxylase antibody (pSer40) (ABIN7540679)

Target: Tyrosine Hydroxylase (TH)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Product Details anti-Tyrosine Hydroxylase Antibody

Binding Specificity: pSer40

Supplier Product No.: 600-401-c99

Supplier: Rockland

Purpose: Tyrosine Hydroxylase phospho S40 Antibody

Cross-Reactivity (Details): Expect reactivity with the following species based on sequence homology: mouse.

Purification: Anti-Tyrosine Hydroxylase pS40 antibody is affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-Tyrosine Hydroxylase pS40 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser 40 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:1000

IF_Microscopy_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Tyrosine Hydroxylase pS40 antibody is tested for use in ELISA, Western Blotting, and ICC/IHC and suitable for IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 42/44 kDa in size corresponding to the tyrosine hydroxylase proteins phosphorylated at Ser40 in the appropriate cell lysate or extract. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine Hydroxylase

Background: Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH,Tyrosine Hydroxylase pS40 antibody recognizes Tyrosine hydroxylase (TH) which is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse. TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling. The activity of TH is also regulated by phosphorylation. Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs. Tyrosine Hydroxylase pS40 antibody is ideal for investigators involved in Neuronal Circuitry and disorders, and more generally in Neuroscience.

Gene ID: 25085

NCBI Accession: NP_036872

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour