Recombinant HMGB1 antibody

Catalog No. ABIN7540813

The Rabbit Monoclonal anti-HMGB1 antibody has been validated for WB, IF, ICC, FACS and IHC (p). It is suitable to detect HMGB1 in samples from Human.

Quick Overview for Recombinant HMGB1 antibody (ABIN7540813)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HMGB1 Antibody

Expression System: Mammalian cells

Purpose: Rabbit recombinant monoclonal antibody raised against HMGB1.

Specificity: This antibody detects endogenous levels of HMGB1 and does not cross-react with related proteins.

Cross-Reactivity: Human, Mouse, Rat

Immunogen: Original antibody is raised against recombinant HMGB1.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry (1:10-1:100),Immunocytochemistry (1:50-1:200),Immunofluorescence (1:50-1:200),Immunohistochemistry (1:50-1:200),Western Blot (1:1000-1:5000),The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In PBS , pH 7.2 (0.02 % sodium azide and 50 % glycerol)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background: High-mobility group box 1

Gene ID: 3146

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome