Recombinant CD11b antibody

Catalog No. ABIN7543093

This anti-CD11b antibody is a Rat Monoclonal antibody detecting CD11b in FACS, IHC, WB, IP, IF, ELISA, ICC, Func and Neut. Suitable for Mouse.

Quick Overview for Recombinant CD11b antibody (ABIN7543093)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Functional Studies (Func), Neutralization (Neut)

Clone: M1-70

Product Details anti-CD11b Antibody

Purpose: Anti-CD11b [M1/70], Rat IgG2b, kappa

Specificity: This antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, and blood monocytes. CD11b is expressed on 8 % of spleen cells, 44 % of bone marrow cells, and less than 1 % of thymocytes, and is commonly used as a microglial marker in nervous tissue.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising DA (dark agouti) and Albino Oxford (AO) strain inbred rats with nylon wool-purified B10 mouse spleen cells according to the protocol by Springer et al. (Eur J Immunol. 1978).

Isotype: IgG2b kappa

Application Details

Application Notes: This antibody has been used in FACS analyses, ELISA, immunoprecipitation, immunoblotting, immunohistochemistry (frozen/paraffin), immunocytochemistry, neutralisation and functional assays in numerous studies, for example, to demonstrate that the Hippo, Wnt/beta-catenin, and Notch pathways interact to regulate liver size and inhibit hepatocellular carcinoma (Kim et al., 2017), to understand the role of PD-1 in maintaining islet-specific tolerance in diabetes-resistant strains (Martinov et al., 2016), to examine the effect of interleukin 4-induced gene-1 (IL4I1) overexpression on the expression of M2 markers and M1-associated cytokines (Yue et al., 2015), to demonstrate that the small intestinal gut-associated lymphoid tissues (GALT) are the major early sites of prion accumulation and neuroinvasion after oral exposure in oral prion disease pathogenesis (Donaldson et al., 2015), to evaluate the role of Wnt signalling in the ageing of haematopoietic stem cells in mice (Florian et al., 2013), to investigate the relative contribution of receptors for advanced glycation end products (RAGE)-dependent and RAGE-independent signalling pathways in diabetic nephropathy (Watson et al., 2012), to dissect the role of CD11b in obesity-associated inflammation (Zheng et al., 2015), and to describe a novel approach to efficiently differentiate large numbers of mature inflammatory monocytes from heterogeneous bone marrow cell suspensions (Francke et al., 2011).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: Mac-1 alpha, CD11 antigen-like family member B, CD11B (p170), cell surface glycoprotein MAC-1 alpha subunit, cell surface glycoprotein MAC-1 subunit alpha, complement component receptor 3 alpha-a, complement receptor type 3, CR-3 alpha chain, integrin alpha-M, leukocyte adhesion receptor MO1, macrophage antigen alpha

UniProt: A1E2I0

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation