Recombinant CD56 antibody

Catalog No. ABIN7543161

The Rabbit Chimeric anti-CD56 antibody is suitable to detect CD56 in samples from Human. It has been validated for FACS, IF and IP.

Quick Overview for Recombinant CD56 antibody (ABIN7543161)

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD56 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: N901

Product Details anti-CD56 Antibody

Purpose: Anti-CD56 [N901], Rabbit IgG, kappa

Specificity:

This antibody binds CD56, present on large granular lymphocytes including natural killer cells. This antibody does not react with monocytes, granulocytes, B cells, erythrocytes, or platelets.

Application Notes original Clone: This antibody does not block the natural killer cell activity and can be used to rapidly purify functional NK cells (PMID:6574190). This antibody was able to precipitate a band at approx. 200-250 kdalton protein in a immunoprecipitation assay. This antibody successfully blocked the binding of anti-NKH1A antibody in an indirect immunofluorescence assay (PMID:3884668). This antibody is also recommended to be used in the study of cell surface antigens of the natural cells (PMID: 3875369). Conjugate of DC1 with murine and humanizsed versions of N901 was found to be potent and effective in selective killing of target cells in culture (PMID: 7545085). THis antibody can also be used for the separation of natural killer cells in a flow cytometry (PMID: 15985539).

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with human chronic myelogenous leukemia (CML) cells.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: CD56

Background: NCAM1, NCAM, NCAM-1, Neural cell adhesion molecule 1

UniProt: P13591