Recombinant CD19 antibody

Catalog No. ABIN7543176

The Mouse Monoclonal anti-CD19 antibody is suitable to detect CD19 in samples from Mouse. It has been validated for FACS, IF and ELISA.

Quick Overview for Recombinant CD19 antibody (ABIN7543176)

Target: CD19 (CD19 Molecule (CD19))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD19 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Clone: MB19-1

Product Details anti-CD19 Antibody

Purpose: Anti-CD19 [MB19-1], Mouse IgG1, kappa

Specificity: This antibody recognizes murine MB19-1 (CD19), a transmembrane protein present in B lymphocytes that regulates its activation. It is required for proper differentiation of B-1 cells.

Cross-Reactivity: Dog

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgA

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising CD19-deficient mice with a cell line of mCD19+ mouse pre-B cells.

Isotype: IgG1 kappa

Application Details

Application Notes: This clone can be used to detect and analyze the presence of the CD19 transmembrane protein. The specificity towards the protein has been confirmed using ELISA and immunofluorescence (Sato et al., 1996, PMID: 8906812). Additionally, the MB19-1 antibody has been used in several flow cytometry assays. One group used this antibody to determine the differences in CD19 distribution between cells of young and old mice (Yamamoto et al., 2018, PMID: 29625072). Another group used it to differentiate eosinophils from other leukocytes (Vanella et al., 2017, PMID: 27043413). A final group used flow cytometry to determine the fraction of B cells cells in a sample with this antibody (Bedaret et al., 2009, PMID: 19907079).

Comment:

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgA format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD19

Target: CD19 (CD19 Molecule (CD19))

Alternative Name: CD19

Background: B-lymphocyte antigen CD19, Differentiation antigen CD19, B-lymphocyte surface antigen B4, B4, CD19 Molecule, CD19 antigen

UniProt: P25918

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway