Recombinant KLRK1 antibody

Catalog No. ABIN7543177

The Rabbit Chimeric anti-KLRK1 antibody (Clone CX-5) (ABIN7543177) specifically detects KLRK1 in FACS, BP and Neut. The antibody is reactive with Mouse samples.

Quick Overview for Recombinant KLRK1 antibody (ABIN7543177)

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This KLRK1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Peptide (BP), Neutralization (Neut)

Clone: CX-5

Product Details anti-KLRK1 Antibody

Purpose: Anti-NKG2D [CX-5], Rabbit IgG, lambda

Specificity: This antibody recognizes mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1 thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original rat IgG1 version of this antibody was raised by immunizing rats against mouse KLRK1.

Isotype: IgG lambda

Application Details

Application Notes: It has been shown that CX5 is a non-depleting antibody, which blocks binding of NKG2D to its ligands and mediates internalization of the receptor. This antibody has been widely used to block NKG2D (Wang et al., 2018, pmid: 29447423, Yang et al., 2017, pmid: 28455530) as well as to detect NKG2D-posotive cells via flow cytometry (Hosomi et al., 2017, pmid: 28747426).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for KLRK1

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Alternative Name: NKG2D

Background: CD314, KLRK1, NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D-activating NK receptor, CX5

UniProt: O54709

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process