Recombinant beta Amyloid antibody

Catalog No. ABIN7543179

The Human Chimeric anti-beta Amyloid antibody has been validated for WB, ELISA, IHC and IP. It is suitable to detect beta Amyloid in samples from Human.

Quick Overview for Recombinant beta Amyloid antibody (ABIN7543179)

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Human

Clonality: Chimeric

Conjugate: This beta Amyloid antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: 21F12

Product Details anti-beta Amyloid Antibody

Purpose: Anti-Amyloid Beta [21F12], Human IgG1, kappa

Specificity: This antibody recognizes amino acids 33-42 of amyloid beta.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing A/J mice with a synthetic peptide (NH2-CYS-NH-CH2-(CH2)5-CO-GLMVGGVVIA-COOH) as an immunogen.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody has very high specificity for Aβ1-42 over Aβ1-40. This antibody is capable of immunoprecipitating Aβ peptides ending at aa position 42 and also p3 peptides (PMID: 8917563). This antibody is also recomended for detection of amyloid beta using sandwich ELISA (PMID: 9037091, 19204155). This antibody was also used in the quantification of the proportion of full-length Aβ using western blotting (PMID: 12787077). This antibody has also been used for the identification of Aβ42 plaques on paraffin frontal sections(PMID: 15215183, 19660650). This antibody is unable to bind plaques in unfixed brain sections. This antibody is also not capable of clearing plaque after in vivo administration (PMID: 10932230).

Comment:

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for beta Amyloid

Target: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Alternative Name: Amyloid Beta

Background: Abeta, A-beta, Aβ, Aβ42, Alzheimer disease amyloid protein, S-APP-beta

UniProt: P05067

Pathways: Inflammasome