Recombinant CD14 antibody

Catalog No. ABIN7543197

The Rabbit Chimeric anti-CD14 antibody has been validated for FACS, WB and IF. It is suitable to detect CD14 in samples from Human.

Quick Overview for Recombinant CD14 antibody (ABIN7543197)

Target: CD14

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Clone: MiL2

Product Details anti-CD14 Antibody

Purpose: Anti-CD14 [MiL2], Rabbit IgG, kappa

Specificity: This antibody is specific for porcine CD14.

Cross-Reactivity: Pig

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was raised by immunizing a mouse with porcine CD14.

Isotype: IgG kappa

Application Details

Application Notes: Western blot was performed using this antibody on proteins (US20170107294). This antibody was used for flow cytometery on fresh human or porcine whole blood (US20170107294) (Thorgersen et al, 2009, pmid:19047409). The biological effect of this antibody on the inflammatory response of piglets was tested (US20170107294). ELISA was performed on C1q and human FcγRs using this antibody (US20170107294). Immunofluorescence was performed on both PBMC and whole blood samples using this antibody (Zelnickova et al, 2007, pmid:17720184). Immunofluorescent staining was performed on cardiac and diaphragmatic lung lobes using this antibody (Gucht et al, 2005, pmid:15802956).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: Monocyte antigen, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades