Recombinant FAP antibody

Catalog No. ABIN7543216

The Rabbit Chimeric anti-FAP antibody (Clone 3F2) (ABIN7543216) specifically detects FAP in IHC, ELISA and FACS. The antibody is reactive with Human, Mouse and Cynomolgus samples.

Quick Overview for Recombinant FAP antibody (ABIN7543216)

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Cynomolgus

Host: Rabbit

Clonality: Chimeric

Conjugate: This FAP antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 3F2

Product Details anti-FAP Antibody

Purpose: Anti-FAP [3F2], Rabbit IgG, kappa

Specificity:

This antibody can bind human, mouse and cynomolgus monkey fibroblast activation protein.

Application Notes original Clone: The original mouse antibody was used to check the expression levels of FAP on the surface of GM05389 lung fibroblasts using flow cytometry. The original mouse IgG1 version of this antibody binds human, mouse and cynomolgus monkey FAP with an avidity of 39 pM, 29 pM and 42 pM respectively. Binding of human IgG1 antibody 3F2 to human and murine FAP expressed on stably transfected HEK293 cells, human fibroblasts cell line GM05389 was measured by floy cytometry. The mouse IgG2a version of this antibody was also used for the immunohistochemical stainings of frozen tissue microarray (TMA) containing 10 invasive ductal carcinomas of the breast, 10 colorectal adenocarcinomas and 10 non-small cell lung cancers (US20200079873).

Cross-Reactivity: Cynomolgus, Mouse

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Purification: Protein A affinity purified

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2a

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for FAP

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: FAP

Background: Seprase, Fibroblast activation protein, Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, FAPalpha, Fibroblast activation protein alpha, Gelatin degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, SIMP, Surface-expressed protease

UniProt: Q12884

Pathways: Tube Formation