Recombinant MUC1 antibody

Catalog No. ABIN7543226

This Rabbit Chimeric antibody specifically detects MUC1 in ELISA, FACS and SPR. It exhibits reactivity toward Human.

Quick Overview for Recombinant MUC1 antibody (ABIN7543226)

Target: MUC1 (Mucin 1 (MUC1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This MUC1 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Surface Plasmon Resonance (SPR)

Clone: 12D10

Product Details anti-MUC1 Antibody

Purpose: Anti-MUC1 [12D10], Rabbit IgG, kappa

Specificity: This antibody is specific for the unsubstituted O-6 position of the Neu5Ac residue of MUC1. The alpha subunit of MUC1 has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit of MUC-1 contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing female Balb/c mice with MUC1 antigen.

Isotype: IgG kappa

Application Details

Application Notes: To characterize this antibody an ELISA was preformed on MUC1 glycorproteins. Furthermore, the binding constant of this antibody was determined by surface plasmon resonance on MUC1 glycoprotein using the mouse version of this antibody. Finally, human breast cancer cells (T-47D), human lung adenocarcinoma cells (Calu-3), human mucoepidermoid carcinoma cells (H292), and human lung adenocarcinoma epithelial cells were used for flow cytometry with the murine version of this antibody (Naito et al, 2017, pmid:30023556).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: MUC1

Background: Mucin-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling