Recombinant Aquaporin 4 antibody

Catalog No. ABIN7543245

The Mouse Monoclonal anti-Aquaporin 4 antibody has been validated for IP, ELISA, FACS and InhA. It is suitable to detect Aquaporin 4 in samples from Human.

Quick Overview for Recombinant Aquaporin 4 antibody (ABIN7543245)

Target: Aquaporin 4 (AQP4)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aquaporin 4 antibody is un-conjugated

Application: Immunoprecipitation (IP), ELISA, Flow Cytometry (FACS), Inhibition Assay (InhA)

Clone: D15107

Product Details anti-Aquaporin 4 Antibody

Purpose: Anti-Aquaporin 4 [D15107], Mouse IgG2a, kappa

Specificity: This antibody recognizes and binds the extracellular domains of both the M1 and M23 isoforms of human aquaporin 4 (AQP4). It also binds the M23 isoform of mouse aquaporin 4, and does not recognize the M1 isoform of mouse aquaporin 4. AQP4 is a water channel expressed on astrocytes throughout the CNS and in skeletal muscle and some epithelial cells outside of the CNS. It plays an important role in brain water homeostasis and in glymphatic solute transport. Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system (CNS) that is associated with demyelination and neurological deficits in optic nerve, spinal cord and brainstem. Most NMOSD patients are seropositive for IgG autoantibodies (NMO-IgG) directed against extracellular epitopes of aquaporin-4 (AQP4). Prevention of the binding of NMO-IgG, therefore, may alleviate the disease.

Cross-Reactivity: Mouse

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing an aquaporin 4 knock out mice with human AQP4 as an immunogen expressed on the surface of baculovirus.

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody binds extracellular domain of human aquaporin 4 with a very high binding affinity. The binding of this antibody inhibits the binding of NMO-IgG to AQP4 (PMID: 23746426). The binding affinity of this antibody for human AQP4 M23 isoform expressed on the surface of baculovirus and CHO cells was tested with ELISA (PMID: 26398585, WO2014196658). The binding characterization of this antibody was done using flow cytometry. This antibody can be used for the immunoprecipitation of human AQP4 but not mouse AQP4 (WO2014196658, PMID: 23909421). This antibody is not suitable for detection of denatured human AQP4 in a western blot assay (WO2014196658). Live imaging using Alexa-Fluor-555-labelled antibodies revealed that this antibody more rapidly bound to cells expressing human aquaporin-4 and strongly enhanced endocytosis and lysosomal degradation of AQP4, especially in cells expressing the M1 isoform alone. Chimeric D15107 prevented complement-dependent cytotoxicity induced by NMO-IgG from patient sera in vitro (PMID: 26398585).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Aquaporin 4

Target: Aquaporin 4 (AQP4)

Alternative Name: Aquaporin 4

Background: AQP4, AQP-4, Aquaporin-4, MIWC, WCH4, Mercurial-insensitive water channel

UniProt: P55087

Pathways: Sensory Perception of Sound