Recombinant MBP antibody (AA 78-88, AA 80-89)

Catalog No. ABIN7543258

The Rabbit Chimeric anti-MBP antibody (Clone 845D3) (ABIN7543258) specifically detects MBP in ELISA and RIA. The antibody is reactive with Human and Cow samples.

Quick Overview for Recombinant MBP antibody (AA 78-88, AA 80-89) (ABIN7543258)

Target: MBP (Myelin Basic Protein (MBP))

Antibody Type: Recombinant Antibody

Reactivity: Human, Cow

Host: Rabbit

Clonality: Chimeric

Conjugate: This MBP antibody is un-conjugated

Application: ELISA, Radioimmunoassay (RIA)

Clone: 845D3

Product Details anti-MBP Antibody

Binding Specificity: AA 78-88, AA 80-89

Purpose: Anti-Myelin basic protein [845D3], Rabbit IgG, kappa

Specificity:

This antibody binds human myelin basic protein (MBP), amino acid residues 80-89 (QDENPVVHF). This antibody also cross reacts with bovine MBP comprising amino acids 78-88. The MBP isoforms 4 and 14 along with PLP are most abundant protein components of the myelin membrane in the CNS. The smaller isoforms are believed to have an important role in remyelination of denuded axons in multiple sclerosis.

Application Notes original Clone: The epitope mapping of this antibody was done using solid phase radioimmunoassay (PMID: 2419434). This antibody can be used in the dection of MBP in a sample using ELISA (PMID: 2466871).

Cross-Reactivity: Cow

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by hyperimmunization of BALB/c mice with human MBP peptide comprising amino acids from 80-89.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for MBP

Target: MBP (Myelin Basic Protein (MBP))

Alternative Name: Myelin basic protein

Background: MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein, 84-5D3

UniProt: P02686