Recombinant XCR1 antibody

Catalog No. ABIN7543269

The Rabbit Chimeric anti-XCR1 antibody is suitable to detect XCR1 in samples from Mouse and Rat. It has been validated for IHC, FACS and in vivo.

Quick Overview for Recombinant XCR1 antibody (ABIN7543269)

Target: XCR1 (Chemokine (C Motif) Receptor 1 (XCR1))

Antibody Type: Recombinant Antibody

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Chimeric

Conjugate: This XCR1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), In vivo Studies (in vivo)

Clone: MARX10

Product Details anti-XCR1 Antibody

Purpose: Anti-XCR1 [MARX10], Rabbit IgG, kappa

Specificity:

This antibody binds mouse XCR1, which is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80 % of CD8+ the dendritic cells. It transduces a signal by increasing the intracellular calcium ions level. This antibody is also reported to cross-reacts with rat CXR1.

Application Notes original Clone: This antibody was used to stain cryostat sections (12 μm) of spleens from C57BL/6 WT and B6.XCR1-lacZ mice. It was found that this antibody could stain 20 % of splenic conventional dendritic cells in C57BL/6 WT mice but did not give a signal in homozygous B6.XCR1-lacZ animals, which lack the XCR1 receptor. This antibody was also used to subsets of dendritic cells that express XCR1 using flow cytometry (Bachem et al., 2012, PMID: 22826713). Digoxigenin (Dig) coupled MARX10 was injected into C57BL/6 mice for in vivo labeling of XCR1⁺ dendritic cells. Recombinant ovalbumin (OVA) coupled MARX10 (MARX10-OVA) was used to measure antibody levels in C57BL/6 and homozygous B6.XCR1-lacZ mice serum following injection and for an in vivo cytotoxicity assay, MARX10-OVA was found to be highly effective in inducing CD8⁺ T cell cytotoxicity in vivo (Hartung et al., 2015, PMID: 25520399). This antibody has been added to the Antibody Registry (RRID: AB_3668949).

Cross-Reactivity: Rat

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing B6.XCR1-lacZ mice every 3 weeks (4 times in succession) with 30 x 10^6 CD11c-enriched cells from C57BL/6 WT mice in combination with heat-inactivated B. pertussis.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2b

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for XCR1

Target: XCR1 (Chemokine (C Motif) Receptor 1 (XCR1))

Alternative Name: XCR1

Background: MXCR1, Ccxcr1, Chemokine XC receptor 1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1, MARX1O

UniProt: Q9R0M1