Recombinant F2RL1 antibody (Cleavage Site)

Catalog No. ABIN7543274

This Rabbit Chimeric antibody specifically detects F2RL1 in ELISA. It exhibits reactivity toward Human.

Quick Overview for Recombinant F2RL1 antibody (Cleavage Site) (ABIN7543274)

Target: F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This F2RL1 antibody is un-conjugated

Application: ELISA

Clone: Ab4999

Product Details anti-F2RL1 Antibody

Binding Specificity: Cleavage Site

Purpose: Anti-PAR2 [Ab4999 (Par-B)], Rabbit IgG, lambda

Specificity: This antibody has binding specificity for N-terminal PAR-2 peptide 'SKGRSLIGKVDGTSHVTGKGV' and can bind human, mouse, rat and cynomolgus monkey PAR-2 peptide. PAR2 acts as a receptor for trypsin and trypsin-like enzymes coupled to G proteins.

Cross-Reactivity: Cynomolgus, Mouse, Rat

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG4

Purification: Protein A affinity purified

Immunogen: The original antibody was generated using phage display techniques to pan for antibodies (Fab fragments) capable of immunospecifically binding to residues spanning the protease cleavage site at the N-terminal of PAR-2. The affinity maturation of the selected parental clone Ab3777 resulted in generation of the antibody.

Isotype: IgG lambda

Application Details

Application Notes: This antibody was generated by the affinity maturation of a parental clone Ab3777. This antibody exhibited concentration-dependent binding to human PAR-2 transfected HEK293 cells with EC50 values of 0.2 nM. ELISA experiments demonstrated specific binding of this antibody to PAR-2 peptide (coupled to Transferrin as carrier). This antibody did not show any binding affinity for PAR-4 peptide or PAR-1 peptide. This antibody showed potent inhibition of calcium release in the human lung epithelial cell line A549 stimulated with trypsin similar to the parental clone Ab3777. (US20110311553).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for F2RL1

Target: F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))

Alternative Name: PAR2

Background: F2RL1, PAR-2, GPR11, Proteinase-activated receptor 2, Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like 1, Ab3777

UniProt: P55085

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome