Recombinant TIGIT antibody

Catalog No. ABIN7543289

This Mouse Monoclonal antibody specifically detects TIGIT in ELISA, FACS and BR. It exhibits reactivity toward Human.

Quick Overview for Recombinant TIGIT antibody (ABIN7543289)

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIGIT antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: 4A5

Product Details anti-TIGIT Antibody

Purpose: Anti-TIGIT [4A5], Mouse IgG1, kappa

Specificity: This antibody binds the extracellular domain of the human TIGIT (T cell immunoreceptor with Ig and ITIM domains). TIGIT is a type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins. It is an immune receptor present on some T cells and natural killer cells (NK). It binds the poliovirus receptor (PVR) with high affinity which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing 9 week old BALB/c mice with recombinant human TIGIT extracellular region Fc fusion protein in complete Freund's adjuvant.

Isotype: IgG1 kappa

Application Details

Application Notes: The binding characterization of this antibody towards human TIGIT was done using ELISA and flow cytometry. This antibody can bind human TIGIT extracellular domain Fc fusion protein in an ELISA and is also capable of recognizing TIGIT expressed on HEK293 cells by flow cytometry. The original IgG1 version of this antibody binds human TIGIT with a binding affinity of Kd=3.732 X 10^(-10) M. This antibody was also reported to successfully block the binding of TIGIT to its ligand CD155. This antibody is capable of binding NK cells in vitro. The anti-cancer activity of this antibody was tested on ovarian cancer cell HO8910, human non-small cell lung cancer cell NCl-H358 and human non-small cell lung cancer cell NCl-H1650 (CN109384846).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: TIGIT

Background: VSTM3, VSIG9, WUCAM, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3

UniProt: Q495A1

Pathways: Cancer Immune Checkpoints