Recombinant TIGIT antibody

Catalog No. ABIN7543291

This anti-TIGIT antibody is a Human Monoclonal antibody detecting TIGIT in ELISA, FACS and BR. Suitable for Human.

Quick Overview for Recombinant TIGIT antibody (ABIN7543291)

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Human

Clonality: Monoclonal

Conjugate: This TIGIT antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: AET2010

Product Details anti-TIGIT Antibody

Purpose: Anti-TIGIT [AET2010], Human IgG1, lambda

Specificity: This antibody binds the human TIGIT (T cell immunoreceptor with Ig and ITIM domains). TIGIT is a type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins. It is an immune receptor present on some T cells and natural killer cells (NK). It binds the poliovirus receptor (PVR) with high affinity which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was isolated from a fully synthetic human single chain antibody phage display library by multiple rounds of bio panning against human TIGIT.

Isotype: IgG1 lambda

Application Details

Application Notes: The binding characterization of this antibody for TIGIT was done using ELISA. The IgG1 version of this antibody binds human TIGIT with a binding affinity of Kd= 3.74 nM. This antibody is also capable of blocking the binding of TIGIT to its ligand CD155. The binding of this antibody to membrane bound TIGIT was determined using flow cytometry. The anti-tumor activity of this antibody was tested against NK-92MI cells in vitro. It was also reported that AET 2010 exhibits enhanced anti-tumor activity in the CDX mouse model of NK-92MI cells (CN112679610).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: TIGIT

Background: VSTM3, VSIG9, WUCAM, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3

UniProt: Q495A1

Pathways: Cancer Immune Checkpoints