Recombinant KRT8 antibody

Catalog No. ABIN7543313

This Mouse Monoclonal antibody specifically detects KRT8 in WB, IHC, ELISA and IP. It exhibits reactivity toward Human.

Quick Overview for Recombinant KRT8 antibody (ABIN7543313)

Target: KRT8 (Keratin 8 (KRT8))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Clone: TS1

Product Details anti-KRT8 Antibody

Purpose: Anti-Cytokeratin 8 [TS1], Mouse IgG1, kappa

Specificity: This antibody binds a discontinuous epitope on helical 2B domain of the human cytokeratin 8 between amino acid residues 340-365 (QRGELAIKDANAKLSELEAALQRAKQ). Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Together with KRT19, cytokeratin 8 helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Cytokeratin 8 is a serological tumor marker

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with purified human gastrointestinal tumor derived cytokeratin.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody has specific reactivity for human cytokeratin 8. The binding characterization and epitope mapping of this antibody was done using ELISA (PMID: 9486565, 9892182). This antibody was also capable of immunoprecipitating human cytokeratin 8 from a sample. This antibody can be used in the identification of cytokeratin 8 in a western blot assay. This antibody was also used in the immunohistochemical staining of frozen sections of normal human adult tissues like skin, esophagus and liver (PMID: 2479674). This antibody is capable of binding cytokeratin 8 expressed on carcinomas in vivo. This antibody was used for the radio immunolocalization of cytokeratin 8 and radioimmunotherapy (PMID: 9892182, 7493362, 9406704).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8

Background: CK8, CK-8, CK 8, KRT8, CYK8, Keratin type II cytoskeletal 8, Cytokeratin-8, Type-II keratin Kb8, TS 1, Mab 202

UniProt: P05787