Recombinant KRT8 antibody

Catalog No. ABIN7543314

The Rabbit Chimeric anti-KRT8 antibody has been validated for WB, IHC, ELISA and IP. It is suitable to detect KRT8 in samples from Human.

Quick Overview for Recombinant KRT8 antibody (ABIN7543314)

Target: KRT8 (Keratin 8 (KRT8))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Clone: TS1

Product Details anti-KRT8 Antibody

Purpose: Anti-Cytokeratin 8 [TS1], Rabbit IgG, kappa

Specificity: This antibody binds a discontinuous epitope on helical 2B domain of the human cytokeratin 8 between amino acid residues 340-365 (QRGELAIKDANAKLSELEAALQRAKQ). Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Together with KRT19, cytokeratin 8 helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Cytokeratin 8 is a serological tumor marker

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with purified human gastrointestinal tumor derived cytokeratin.

Isotype: IgG kappa

Application Details

Application Notes: This antibody has specific reactivity for human cytokeratin 8. The binding characterization and epitope mapping of this antibody was done using ELISA (PMID: 9486565, 9892182). This antibody was also capable of immunoprecipitating human cytokeratin 8 from a sample. This antibody can be used in the identification of cytokeratin 8 in a western blot assay. This antibody was also used in the immunohistochemical staining of frozen sections of normal human adult tissues like skin, esophagus and liver (PMID: 2479674). This antibody is capable of binding cytokeratin 8 expressed on carcinomas in vivo. This antibody was used for the radio immunolocalization of cytokeratin 8 and radioimmunotherapy (PMID: 9892182, 7493362, 9406704).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Cytokeratin 8

Background: CK8, CK-8, CK 8, KRT8, CYK8, Keratin type II cytoskeletal 8, Cytokeratin-8, Type-II keratin Kb8, TS 1, Mab 202

UniProt: P05787