Recombinant MSH2 antibody

Catalog No. ABIN7543328

This Mouse Monoclonal antibody specifically detects MSH2 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant MSH2 antibody (ABIN7543328)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 1B11-14-16

Product Details anti-MSH2 Antibody

Purpose: Anti-MSH2 [1B11-14-16], Mouse IgG2b, kappa

Specificity: This antibody binds an epitope on the human DNA mismatch protein MSH2 between amino acids 919-934 (NSFVNEIISRIKVTT). MSH2 forms a heterodimer with MSH6 to make the human MutSα mismatch repair complex. The DNA mismatch repair system (MMR) is composed of enzyme molecules that specifically repair DNA base mismatches. MSH2 is involved in many different forms of DNA repair, including transcription-coupled repair, homologous recombination, and base excision repair. Studies have shown that mutations and/or loss of function of MMR genes are related to the occurrence of a variety of tumors, such as colorectal cancer, skin cancer, ovarian cancer, and endometrial cancer.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with human MSH2 protein peptide (KLH-NSFVNEIISRIKVTT-Cys) conjugated with KLH in equal volume of Freund's complete adjuvant.

Isotype: IgG2b kappa

Application Details

Application Notes: The binding specificity of this antibody for human MSH2 was determined using ELISA. This antibody was capable of detecting MSH2 from HCT8 and Biu-87 cell lysates in a western blot. This antibody was also used in the immunohistochemical analysis of normal tissues like tonsil, appendix tissues and cancerous tissues like colorectal and bladder cancer. This antibody shows similar staining of MSH2 protein compared to the commercially available antibody G219-1129 (CN110218251).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Alternative Name: MSH2

Background: HMSH2, DNA mismatch repair protein Msh2, MutS protein homolog 2, CGMCC NO.17405, G219-1129

UniProt: P43246

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response