Recombinant Glypican 3 antibody (Extracellular Domain)

Catalog No. ABIN7543338

The Rabbit Chimeric anti-Glypican 3 antibody has been validated for FACS, WB, IHC and ELISA. It is suitable to detect Glypican 3 in samples from Human.

Quick Overview for Recombinant Glypican 3 antibody (Extracellular Domain) (ABIN7543338)

Target: Glypican 3 (GPC3)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This Glypican 3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 7H1

Product Details anti-Glypican 3 Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-Glypican 3 [7H1], Rabbit IgG, kappa

Specificity: This antibody binds an epitope between amino acids 25 to 137 of the human glypican 3 (GPC3). This antibody cross reacts with cynomolgus monkey, rat and mouse GPC3. Glypican-3 is an oncofetal antigen that belongs to the glypican family of glycosyl-phosphatidylinositol-anchored heparin sulfate proteoglycans. It negatively regulates the hedgehog signaling pathway and positively regulates the canonical Wnt signaling pathway. Multiple studies have revealed that GPC-3 is overexpressed in hepatocellular carcinoma (HCC).

Cross-Reactivity: Cynomolgus, Mouse, Rat

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with a recombinant human GPC3 extracellular domain (ECD, amino acids of 1-547) fused to a C-terminal Flag (RADYKDDDDK).

Isotype: IgG kappa

Application Details

Application Notes: This antibody can bind human glypican 3 expressed on the surface of 293S cells, HepG2 X1 cells and JHH7 cells in a flow cytometric assay. The binding characterization of this antibody to huGPC3, cynoGPC3, and HepG2 cells was done using ELISA and immunohistochemistry. This antibody strongly reacts with hepatic cancer tissue microarray, JHH cells, HepG2 cells, and cells stably transfected with GPC3 in immunohistochemistry. This antibody also detects human, cynomolgus monkey, rat, and mouse GPC3 by western blot. This antibody was also used in the generation of an antibody drug conjugate with maleimide acetal PNU-159682 or monomethyl disulfide N10-linked PBD. The ADCC activity of the antibody drug conjugate was investigated using a human HepG2 X1 and JHH7 xenograft model and substantial tumor growth inhibition was achieved with 7H1-disulfide-PBD (US9926377).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Glypican 3

Target: Glypican 3 (GPC3)

Alternative Name: Glypican 3

Background: GPC3, Glypican-3, GPC-3, GTR2-2, MXR7, OCI5, DGSX, Intestinal protein OCI-5

UniProt: P51654

Pathways: Glycosaminoglycan Metabolic Process