Recombinant CTLA4 antibody

Catalog No. ABIN7543361

This Mouse Monoclonal antibody specifically detects CTLA4 in ELISA and in vivo. It exhibits reactivity toward Human.

Quick Overview for Recombinant CTLA4 antibody (ABIN7543361)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: ELISA, In vivo Studies (in vivo)

Clone: 5E3

Product Details anti-CTLA4 Antibody

Purpose: Anti-CTLA-4 [5E3], Mouse IgG1, kappa

Specificity:

The antibody is specific for CTLA-4. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses.

Application Notes original Clone: The specificity of the antibody was confirmed by ELISA analysis. The kinetic parameters of human CTLA-4 protein binding to the antibody were measured and they resulted to be at the sub-nm level. The antitumor activity of the humanized antibody was demonstrated in vivo in animal models. It was proved that the antibody inhibited the tumor in a dose-dependent manner. Mouse PD-1 antibody was combined with the antibody and used to evaluate the toxicity to mice, these experiments compared with the use of Ipilimumab in combination with PD-1 antibody. Results showed that after 5E3 antibody was used in combination with PD-1 antibody, the immunity to the body and the toxicity of heart, liver and lung are significantly lower than the toxicity of Ipilimumab in combination with PD-1 antibody (CN110343180).

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with CTLA-4 protein.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA-4

Background: Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CD152

UniProt: P16410

Pathways: Cancer Immune Checkpoints