Recombinant K-RAS antibody (Glu12Asp Mutant)

Catalog No. ABIN7543442

This Mouse Monoclonal antibody specifically detects K-RAS in ELISA. It exhibits reactivity toward Human.

Quick Overview for Recombinant K-RAS antibody (Glu12Asp Mutant) (ABIN7543442)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This K-RAS antibody is un-conjugated

Application: ELISA

Clone: D113

Product Details anti-K-RAS Antibody

Binding Specificity: Glu12Asp Mutant

Purpose: Anti-KRAS G12D mutant [D113], Mouse IgG1, kappa

Specificity: This antibody binds the G12D mutant form of human KRAS protein. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic KRAS mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer. KRAS mutation is G12D which is estimated to be present in up to 37 % pancreatic cancers and over 12 % of colorectal cancers.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with a synthetic dodecapeptide 'KLVVVGADGVGK'.

Isotype: IgG1 kappa

Application Details

Application Notes: The binding reactivity and epitope characterization of this antibody towards human KRAS G12D mutant was determined using ELISA (PMID: 9440050). This antibody can bind both the peptide 'KLVVVGADGVGK' conjugated BSA as well as KRAS G12D mutant in an ELISA.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for K-RAS

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: KRAS

Background: K-Ras, GTPase Kras, KRAS2, RASK2, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, ras p21 protein, p21 ras, D113-2-1

UniProt: P01116-2