HA-Tag antibody (Biotin)

Catalog No. ABIN722798

There are 2+ publications for this product available. The Mouse Monoclonal anti-HA-Tag antibody is suitable to detect HA-Tag in samples from Tag. It has been validated for WB.

Quick Overview for HA-Tag antibody (Biotin) (ABIN722798)

Target: HA-Tag

Reactivity: Tag

Host: Mouse

Clonality: Monoclonal

Conjugate: This HA-Tag antibody is conjugated to Biotin

Application: Western Blotting (WB)

Clone: 12CA5

Product Details anti-HA-Tag Antibody (Biotin)

Sequence: YPYDVPDYA

Cross-Reactivity: Tag

Cross-Reactivity (Details): HA tag

Purification: Purified by Protein G.

Immunogen: KLH conjugated to HA tag

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

References for anti-HA-Tag antibody (Biotin) (ABIN722798)

Hager, Forsich, Duchoslav, Burgstaller, Stifter, Weghuber, Lanzerstorfer: "Microcontact Printing of Biomolecules on Various Polymeric Substrates: Limitations and Applicability for Fluorescence Microscopy and Subcellular Micropatterning Assays." in: ACS applied polymer materials, Vol. 4, Issue 10, pp. 6887-6896, (2022) (PubMed).

Lanzerstorfer, Müller, Gordiyenko, Weghuber, Niemeyer: "Highly Modular Protein Micropatterning Sheds Light on the Role of Clathrin-Mediated Endocytosis for the Quantitative Analysis of Protein-Protein Interactions in Live Cells." in: Biomolecules, Vol. 10, Issue 4, (2021) (PubMed).

Target Details for HA-Tag

Target: HA-Tag

Alternative Name: HA tag

Target Type: Tag

Background: Optional[synonyms]: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.