Osteopontin antibody (AA 221-280) (HRP)

Catalog No. ABIN723809

The Rabbit Polyclonal anti-Osteopontin antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect Osteopontin in samples from Mouse and Rat.

Quick Overview for Osteopontin antibody (AA 221-280) (HRP) (ABIN723809)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteopontin antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Osteopontin Antibody (HRP)

Binding Specificity: AA 221-280

Cross-Reactivity: Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse OPN

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: Osteopontin

Background:

Synonyms: OP, 2AR, Bsp, Eta, Opn, Ric, BNSP, BSPI, Opnl, Apl-1, ETA-1, Spp-1, Osteopontin, Bone sialoprotein 1, Calcium oxalate crystal growth inhibitor protein, Early T-lymphocyte activation 1 protein, Minopontin, Secreted phosphoprotein 1, Spp1

Background: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-1 and is essential in the pathway that leads to type I immunity.

Gene ID: 20750

UniProt: P10923

Pathways: Regulation of Cell Size