Choline Acetyltransferase antibody (AA 101-200)

Catalog No. ABIN724070

The Rabbit Polyclonal anti-Choline Acetyltransferase antibody has been validated for ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect Choline Acetyltransferase in samples from Human, Mouse, Rat and Dog. There are 4+ publications available.

Quick Overview for Choline Acetyltransferase antibody (AA 101-200) (ABIN724070)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Choline Acetyltransferase Antibody

Binding Specificity: AA 101-200

Cross-Reactivity: Dog, Human, Mouse, Rat

Predicted Reactivity: Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ChAT

Isotype: IgG

Application Details

Application Notes: ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Choline Acetyltransferase antibody (ABIN724070)

Zhang, Zheng, Geng, Xue, Wang, Xie, Wang, Zhang, Hou: "MicroRNA profiling of atrial fibrillation in canines: miR-206 modulates intrinsic cardiac autonomic nerve remodeling by regulating SOD1." in: PLoS ONE, Vol. 10, Issue 3, pp. e0122674, (2015) (PubMed).

Wieck, El-Nachef, Hou, Spurrier, Holoyda, Schall, Garcia Mojica, Collins, Trecartin, Cheng, Frykman, Grikscheit: "Human and Murine Tissue-Engineered Colon Exhibit Diverse Neuronal Subtypes and Can Be Populated by Enteric Nervous System Progenitor Cells When Donor Colon Is Aganglionic." in: Tissue engineering. Part A, (2015) (PubMed).

Zhang, Jin, Li, Guan, Han, Zhang: "Catalpol improves cholinergic function and reduces inflammatory cytokines in the senescent mice induced by D-galactose." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 58, pp. 50-5, (2013) (PubMed).

Zhu, Zhao, Luo, Li: "Gastrointestinal dysfunction in a Parkinson's disease rat model and the changes of dopaminergic, nitric oxidergic, and cholinergic neurotransmitters in myenteric plexus." in: Journal of molecular neuroscience : MN, Vol. 47, Issue 1, pp. 15-25, (2012) (PubMed).

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: ChAT

Background:

Synonyms: CMS1A, CMS1A2, CHOACTASE, Choline O-acetyltransferase, ChAT, Choline acetylase

Background: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Gene ID: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development