Insulin antibody (AA 46-59)

Catalog No. ABIN724265

The Rabbit Polyclonal anti-Insulin antibody has been validated for WB, ELISA, IHC (p), FACS, IF (p), IF (cc) and IHC (fro). It is suitable to detect Insulin in samples from Mouse and Rat. There is 1 publication available.

Quick Overview for Insulin antibody (AA 46-59) (ABIN724265)

Target: Insulin (INS)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Insulin Antibody

Binding Specificity: AA 46-59

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Insulin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Insulin antibody (ABIN724265)

Yang, Zhao, Zhao, Yuan, Chen, Varghese, Moorhead, Chen, Ruan: "Paradoxical effect of rapamycin on inflammatory stress-induced insulin resistance in vitro and in vivo." in: Scientific reports, Vol. 5, pp. 14959, (2016) (PubMed).

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: Insulin

Background:

Synonyms: ILPR, IRDN, IDDM1, IDDM2, MODY1, Insulin, INS

Background: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Gene ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation