TNF alpha antibody (AA 86-150)

Catalog No. ABIN724580

The Rabbit Polyclonal anti-TNF alpha antibody has been validated for WB, ELISA, IHC (p), IF (p), IHC (fro) and IF (cc). It is suitable to detect TNF alpha in samples from Human, Mouse, Rat, Pig and Rabbit. There are 2+ publications available.

Quick Overview for TNF alpha antibody (AA 86-150) (ABIN724580)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human, Mouse, Rat, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-TNF alpha Antibody

Binding Specificity: AA 86-150

Cross-Reactivity: Human, Mouse, Pig, Rabbit, Rat

Predicted Reactivity: Dog,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human TNF alpha

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-TNF alpha antibody (ABIN724580)

Xu, Huang, Xie, Lin, Guo, Zhuang, Lin, Zhou, Li, Huang, Wei, Man, Zhang: "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." in: BMC cancer, Vol. 13, pp. 247, (2013) (PubMed).

Li, Dan, Hu, Ouzhu, Ciren, Wang, Wang, Yang, Ze: "CD14 overexpression upregulates TNF-?-mediated inflammatory responses and suppresses the malignancy of gastric carcinoma cells." in: Molecular and cellular biochemistry, Vol. 376, Issue 1-2, pp. 137-43, (2013) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background:

Synonyms: DIF, TNFA, TNFSF2, TNF-alpha, Tumor necrosis factor, Cachectin, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNF

Background: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Molecular Weight: 17/26kDa

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome