Serum antibody (HRP)

Catalog No. ABIN7565863

This Rabbit Polyclonal antibody specifically detects Serum in ELISA, WB, IHC and DB. It exhibits reactivity toward Cow.

Quick Overview for Serum antibody (HRP) (ABIN7565863)

Target: Serum

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Product Details

Purpose: Bovine Serum Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and anti-Peroxidase.

Characteristics: Synonyms: Rabbit anti-Bovine Serum Peroxidase Conjugated Antibody, Rabbit anti-Bovine Serum Antibody HRP Conjugation

Purification: Bovine serum is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Bovine serum proteins

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Bovine Serum peroxidase conjugate has been tested by dot blot and is suitable to be assayed against 1.0 μg of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: User Optimized ELISA Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Serum

Background: Background: Anti-Bovine serum antibody detects bovine serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Bovine serum antibody is ideal for investigators involved in Cell Signaling, Cellular Biology and Signal Transduction research.