Serum antibody (HRP)

Catalog No. ABIN7565886

The HRP-conjugated Rabbit Polyclonal anti-Serum antibody (ABIN7565886) specifically detects Serum in ELISA, WB, IHC and DB. The antibody is reactive with Guinea Pig samples.

Quick Overview for Serum antibody (HRP) (ABIN7565886)

Target: Serum

Reactivity: Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Product Details

Purpose: Guinea Pig Serum Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum.

Characteristics: Synonyms: Rabbit anti-Guinea Pig IgG F(ab')2 Fluorescein Conjugated Antibody, Rabbit anti-Guinea Pig IgG F(ab')2 FlTC Conjugated Antibody, Rabbit anti-Guinea Pig IgG Fab2 Fluorescein Conjugated Antibody, Rabbit anti-Guinea Pig IgG Fab2 FlTC Conjugated Antibody

Purification: Guinea Pig Serum is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Guinea Pig serum proteins

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Guinea Pig Serum Peroxidase Conjugated has been tested by dot blot and is suitable to be assayed against 1.0 μg of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: User Optimized ELISA Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Reconstitution Volume: 100 μL

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: Serum

Background: Background: Anti-Guinea Pig Peroxidase antibody detects guinea pig serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Guinea Pig Serum antibody is ideal for investigators involved in Cell Signaling, Cellular Biology and Signal Transduction research.