CD44 antibody (C-Term)

Catalog No. ABIN7565870

The Rabbit Polyclonal anti-CD44 antibody is suitable to detect CD44 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for CD44 antibody (C-Term) (ABIN7565870)

Target: CD44

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CD44 Antibody

Binding Specificity: C-Term

Purpose: CD44 Antibody

Cross-Reactivity (Details): Anti-CD44 Antibody is directed against human CD44 protein.

Characteristics: Synonyms: rabbit anti-CD44 Antibody, CD 44, CD-44 antibody, CDw44, HCAM, homing cell adhesion molecule, lymphocyte homing receptor, LHR, ECM-III, Epican, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, HUTCH-1, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Pgp-1, phagocytic glycoprotein-1, Phagocytic glycoprotein 1, PGP1, Phagocytic glycoprotein I, MDU2, MDU3, MIC4

Purification: CD44 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-CD44 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminal region of human CD44.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Anti-CD44 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 81.5 kDa in Western Blots of specific cell lysates and tissues. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:50,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background: Background: CD44 was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). CD44 is a receptor for hyaluronic acid (HA), an integral component of the extracellular matrix. CD44 mediates cell-cell and cell-matrix interactions through its affinity for HA, and can also interact with ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). The multiple protein isoforms are encoded by a single gene by alternative splicing and are further modified by a range of post-translational modifications. CD44 function is controlled by these posttranslational modifications. The major physiological role of CD44 is to maintain organ and tissue structure via cell-cell and cell-matrix adhesion, but certain variant isoforms can also mediate lymphocyte activation and homing, and the presentation of chemical factors and hormones. CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. CD44 is a multi-structural and multi-functional cell surface molecule involved in cell proliferation, cell differentiation, cell migration, angiogenesis, presentation of cytokines, chemokines, and growth factors to the corresponding receptors, and docking of proteases at the cell membrane, as well as in signaling for cell survival. All these biological properties are essential to the physiological activities of normal cells, but they are also associated with the pathologic activities of cancer cells. CD44, particularly its variants, may be useful as a diagnostic or prognostic marker of malignancy and, in at least some human cancers, it may be a potential target for cancer therapy.

Gene ID: 960

NCBI Accession: NP_000601

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling