TLR4 antibody (N-Term)

Catalog No. ABIN7565942

The Rabbit Polyclonal anti-TLR4 antibody has been validated for WB, IHC and ELISA. It is suitable to detect TLR4 in samples from Human.

Quick Overview for TLR4 antibody (N-Term) (ABIN7565942)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-TLR4 Antibody

Binding Specificity: N-Term

Purpose: TLR4 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human TLR4 protein.

Characteristics: Synonyms: Rabbit Anti-TLR4 Antibody, ARMD10 antibody, CD284 antibody, Toll (Drosophila) homolog antibody, HToll, Homolog of Drosophila Toll, TLR-4 Antibody, TOLL antibody, Toll Endotoxin Hyporesponsiveness antibody, Toll like receptor 4 antibody, Toll like receptor 4 precursor antibody

Purification: The product was purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the n-terminus of human TLR4 protein.

Immunogen Type: Conjugated Peptide

Application Details

Application Notes: Application Note: Anti-TLR4 affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 96 kDa in size corresponding to TLR4 protein in overexpressed lysate by western blotting in the appropriate cell lysate or extract. Immunohistochemistry Dilution: 1:100 - 1:200 Western Blot Dilution: 1:1000 ELISA Dilution: 5 μg/mL Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4

Background: Background: TLR4 (Toll-Like Receptor 4). Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses.  These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kB.  Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide.  Consequently, these mice also showed increased susceptibility to Gram-negative sepsis. Mutations in this gene have been associated with differences in LPS responsiveness, macular degeneration, and pertussis. Anti-TLR4 Antibody is useful for researchers interested in transcription factor research, cytokine research, MIF action through Endocytic Pathway, and rheumatoid arthritis.

Gene ID: 7099, 19924149

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins