His Tag antibody (Atto 550)

Catalog No. ABIN7566000

This anti-His Tag antibody is a Mouse Monoclonal antibody detecting His Tag in WB, FM and FLISA. Suitable for .

Quick Overview for His Tag antibody (Atto 550) (ABIN7566000)

Target: His Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is conjugated to Atto 550

Application: Western Blotting (WB), Fluorescence Microscopy (FM), FLISA

Clone: 33D10-D2-G8

Product Details anti-His Tag Antibody (Atto 550)

Purpose: 6X His Tag ATTO 550 Conjugated Antibody

Cross-Reactivity (Details): 6X HIS Epitope Tag ATTO 550 conjugated antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.

Characteristics: Synonyms: mouse anti-6X His Tag ATTO 550 conjugated Antibody, ATTO 550 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody, AT550, ATTO 550, ATTO-TEC 550

Immunogen:

Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 kappa

Labeling Ratio: 1.0

Application Details

Application Notes: Application Note: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope.  The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins.  This antibody has been tested by western blotting against both the immunizing peptide and His-containing recombinant proteins.  ATTO 550s are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Western Blot Dilution: >1:10,000 FLISA Dilution: >1:20,000 IF Microscopy Dilution: >1:5,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Reconstitution Volume: 50μL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for His Tag

Target: His Tag

Alternative Name: 6X His

Target Type: Tag

Background: Background: 6X His Tag ATTO 550 conjugated Antibody as well as other Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein's biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.