IDH1 antibody (R132H)

Catalog No. ABIN7566428

The Mouse Monoclonal anti-IDH1 antibody has been validated for IHC and ELISA. It is suitable to detect IDH1 in samples from Human.

Quick Overview for IDH1 antibody (R132H) (ABIN7566428)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IDH1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Clone: AG-IHC132

Product Details anti-IDH1 Antibody

Binding Specificity: R132H

Purpose: anti-Isocitrate Dehydrogenase 1 [IDH1] (R132H Mutant) (human), mAb (AG-IHC132)

Characteristics:

Monoclonal Antibody. Recognizes human IDH1 mutated at R132H. Applications: ELISA, IHC. Isotype: Mouse IgG1. Clone: AG-IHC132. Liquid. In Tris Buffer, pH 7.4, containing 1 % BSA and <0.1 % sodium azide. Isocitrate Dehydrogenase 1 (IDH1) is a soluble, cytosolic enzyme involved in the TCA metabolic cycle. The most notable mutation in this enzyme, R132H, is clinically indicated in the majority of astrocytomas and oligodendroglial tumors, with the mutation being associated with more favourable prognosis and increased survival in those patients. IDH1 R132H is also useful in the differential diagnosis between anaplastic glioma and glioblastoma. This antibody is intended to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods. It has been optimized and validated using the BOND-MAX fully automated IHC&ISH stainer (see Protocol).

Isocitrate Dehydrogenase 1 (IDH1) is a soluble, cytosolic enzyme involved in the TCA metabolic cycle. The most notable mutation in this enzyme, R132H, is clinically indicated in the majority of astrocytomas and oligodendroglial tumors, with the mutation being associated with more favourable prognosis and increased survival in those patients. IDH1 R132H is also useful in the differential diagnosis between anaplastic glioma and glioblastoma. This antibody is intended to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods. It has been optimized and validated using the BOND-MAX fully automated IHC&ISH stainer (see Protocol).

Purification: Protein A/G purified.

Immunogen: Synthetic IDH1 peptide.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In Tris Buffer, pH 7.3 - 7.7, containing 1 % BSA and <0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment:

Stable for at least 1 year after receipt when stored at +4°C.

Target Details for IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Alternative Name: Isocitrate Dehydrogenase 1 [IDH1]

UniProt: O75874

Pathways: Warburg Effect