TNF alpha antibody

Catalog No. ABIN7566475

This Rat Monoclonal antibody specifically detects TNF alpha in FACS and Func. It exhibits reactivity toward Mouse.

Quick Overview for TNF alpha antibody (ABIN7566475)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: V1q

Product Details anti-TNF alpha Antibody

Purpose: anti-TNF-alpha (mouse), mAb (blocking) (V1q) (preservative free)

Characteristics:

Monoclonal Antibody. Recognizes mouse TNF-alpha. Reacts with both natural and recombinant mouse TNF-alpha. It does not cross-react with human TNF-alpha and TNF-beta (Lymphotoxin-alpha). Application: FACS, FUNC. Clone: V1q. Isotype: Rat IgG. Liquid. In PBS. Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-α) is a cytokine involved in systemic inflammation and metabolism and is a member of a group of cytokines that stimulate the acute phase reaction. It causes tumor necrosis in vivo and shows also cytolytic activity against tumors. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors 1 and 2. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-α, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-α. The monoclonal antibody V1q recognizes endogenous mouse Tumor Necrosis Factor-α.

Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and metabolism and is a member of a group of cytokines that stimulate the acute phase reaction. It causes tumor necrosis in vivo and shows also cytolytic activity against tumors. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors 1 and 2. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-alpha, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-alpha. The monoclonal antibody V1q recognizes endogenous mouse TNF-alpha.

Purification: Puified

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Immunogen: Cytotoxin purified from ConA induced T cell clone 29.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS.

Preservative: Without preservative

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

Stable for at least 1 year after receipt when stored at -20°C.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-alpha

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome