alpha Tubulin antibody (AA 375-448)

Catalog No. ABIN725630

The Rabbit Polyclonal anti-alpha Tubulin antibody has been validated for WB, ELISA, IHC (p), FACS and IF (p). It is suitable to detect alpha Tubulin in samples from Human, Mouse, Rat and Plant. There are 2+ publications available.

Quick Overview for alpha Tubulin antibody (AA 375-448) (ABIN725630)

Target: alpha Tubulin (TUBA1)

Reactivity: Human, Mouse, Rat, Plant

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-alpha Tubulin Antibody

Binding Specificity: AA 375-448

Specificity: Will recognize many forms of alpha tubulin, Tubulin alpha-1A chain, Tubulin alpha-4A chain etc

Cross-Reactivity: Human, Mouse, Plant, Rat

Predicted Reactivity: Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Tubulin-alpha 1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-alpha Tubulin antibody (ABIN725630)

Wang, Guan, Lu, Jin, Cai, Wang, Wang: "Clinical and tumor significance of tropomyosin-1 expression levels in renal cell carcinoma." in: Oncology reports, Vol. 33, Issue 3, pp. 1326-34, (2015) (PubMed).

Fang, Jiang, Yu, Ye, Wan: "Differentially expression of Tua1, a tubulin-encoding gene, during flowering of tea plant Camellia sinensis (L.) O. Kuntze using cDNA amplified fragment length polymorphism technique." in: Acta biochimica et biophysica Sinica, Vol. 38, Issue 9, pp. 653-62, (2006) (PubMed).

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Tubulin-alpha

Background:

Synonyms: TUBA1, H2-ALPHA, Tubulin alpha-4A chain, Alpha-tubulin 1, Testis-specific alpha-tubulin, Tubulin H2-alpha, Tubulin alpha-1 chain, TUBA4A

Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Gene ID: 7277

UniProt: P68366

Pathways: Microtubule Dynamics