AXL antibody (Extracellular, N-Term)

Catalog No. ABIN7581829

This Rabbit Polyclonal antibody specifically detects AXL in WB, IHC, FACS and LCI. It exhibits reactivity toward Human.

Quick Overview for AXL antibody (Extracellular, N-Term) (ABIN7581829)

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AXL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-AXL Antibody

Binding Specificity: AA 368-382, Extracellular, N-Term

Purpose: A Rabbit Polyclonal antibody to AXL (extracellular)

Sequence: (C)RLAYQGQDTP EVLMD

Predicted Reactivity: Mouse,Rat - 14 out of 15 amino acids residues identical

Characteristics: Anti-AXL (extracellular) Antibody (ABIN7581829) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize AXL from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RLAYQGQDTPEVLMD, corresponding to amino acid residues 368 - 382 of human AXL

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 5 mg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for AXL

Target: AXL (AXL Receptor tyrosine Kinase (AXL))

Alternative Name: AXL 

Background:

Synonyms: AXL Receptor Tyrosine Kinase, Tyrosine-Protein Kinase Receptor UFO, AXL Oncogene, Tyro7, UFO

Description: AXL, also known as Tyrosine Kinase Receptor UFO and Tyro7, is a member of the receptor tyrosine kinase TAM family, that includes the receptors Tyro3, AXL and MERTK 1,2.The TAM family receptors are widely expressed in normal cells and tissues, such as monocytes, platelets, endothelial cells, brain and heart, where they regulate cell survival, non-inflammatory clearance of apoptotic cells by phagocytic cells, natural killer cell differentiation, platelet aggregation, and more 1,2.The TAM family receptors show structural similarities with other tyrosine kinase receptors, that is, an extracellular N-terminal domain containing two immunoglobulin-like and two fibronectin 3 domains, followed by a hydrophobic single pass transmembrane domain, and a cytoplasmic C-terminal tail containing a tyrosine kinase domain1,2.Axl and the other TAM receptors can be activated via their ligands, growth arrest specific 6 protein (Gas6) and Protein S (Pros1), which are members of the family of vitamin K-dependent proteins 3.Both AXL receptor and its high affinity ligand Gas6, are key regulators of immune cell activation, and have been shown to be expressed in cancer cells where they promote survival and invasion and contribute to resistance to various therapies 3,4,5. AXL expression is linked to increased risk of metastasis and poor survival in a variety of solid cancers including breast cancer, non-small cell lung carcinoma, ovarian cancer, and clear cell renal carcinoma 3,4. AXL activation in cancer cells and various stromal cells also results in tumor microenvironment deregulation, leading to modulation of angiogenesis, fibrosis, immune response and hypoxia 3,4.Based on these findings, AXL has been identified as a critical therapeutic target for solid cancers, and indeed several therapeutic modalities including small molecules and monoclonal antibodies, are currently being developed 2, 4.

Gene ID: 558

UniProt: P30530

Pathways: RTK Signaling, Cellular Response to Molecule of Bacterial Origin