C3AR1 antibody (Extracellular)

Catalog No. ABIN7581833

The Rabbit Polyclonal anti-C3AR1 antibody has been validated for WB, IHC, FACS and LCI. It is suitable to detect C3AR1 in samples from Mouse.

Quick Overview for C3AR1 antibody (Extracellular) (ABIN7581833)

Target: C3AR1 (Complement Component 3a Receptor 1 (C3AR1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C3AR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-C3AR1 Antibody

Binding Specificity: AA 172-186, Extracellular

Purpose: A Rabbit Polyclonal antibody to C3aR1 (extracellular)

Sequence: CRYNFDSSRS YDYWD

Specificity: Extracellular, 2nd loop.

Predicted Reactivity: Rat - 13 out of 15 amino acid residues identical It will not recognize C3aR1 from human samples

Characteristics: Anti-C3aR1 (extracellular) Antibody (ABIN7581833) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize C3aR1 from mouse and rat samples. The antibody will not recognize C3aR1 from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CRYNFDSSRSYDYWD, corresponding to amino acid residues 172 - 186 of mouse C3aR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for C3AR1

Target: C3AR1 (Complement Component 3a Receptor 1 (C3AR1))

Alternative Name: C3AR1

Background: Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor,The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages1. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex1.Three different pathways are known to activate the complement. The C3 complement plays a key role in all three pathways leading to the activation of the complement. Produced in the liver, C3 is the most abundant complement protein in the serum. It is composed of two polypeptides, an α and β chain. Cleavage of C3 leads to the production of C3b and C3a1.C3a binds to C3a receptor, a G-protein coupled receptor which couples to Gi upon activation. Like all other members of the superfamily, C3a receptor has seven transmembrane domains with an extracellular N-terminus and intracellular C-terminal tail1. The receptor has two glycosylation sites, an unusually large extracellular loop with a sulfated tyrosine residue important for ligand recognition and binding2,3.C3a receptor is expressed in cells of myeloid origin like neutrophils, macrophages and dendritic cells to name a few2. It is also detected in astrocytes from inflamed brain4, endothelial cells5 and activated human T cells6. mRNA of C3a receptor is detected in lung, liver, kidney, brain heart, muscle and testis2,7.

Gene ID: 12267

UniProt: O09047

Pathways: Complement System