GFAP antibody (Glial Fibrillary Acidic Protein) (AA 30-80)

Details for Product anti-GFAP Antibody No. ABIN726200, Supplier: Log in to see
Antigen
  • GFAP
  • AI836096
  • cb345
  • etID36982.3
  • gfapl
  • wu:fb34h11
  • wu:fk42c12
  • xx:af506734
  • zgc:110485
  • glial fibrillary acidic protein
  • GFAP
  • LOC100136168
  • gfap
  • Gfap
Epitope
AA 30-80
104
50
31
30
23
15
15
12
11
9
6
6
6
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
829
522
506
254
249
196
162
32
30
27
23
22
19
18
13
11
8
8
3
3
2
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
548
343
26
26
4
3
1
Clonality
Polyclonal
Conjugate
This GFAP antibody is un-conjugated
43
31
30
19
18
16
12
9
9
8
6
6
6
6
6
6
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
735
437
321
260
221
219
153
116
74
67
66
53
16
12
12
9
7
5
4
3
2
2
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen KLH conjugated synthetic peptide derived from human GFAP
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others GFAP products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name GFAP (GFAP Antibody Abstract)
Background

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Subcellular location: Cytoplasm

Synonyms: Glial fibrillary acidic protein, GFAP

Gene ID 2670
UniProt P14136
Application Notes WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 30-80) antibody (ABIN726200) Formalin-fixed and paraffin embedded: rat brain tissue Anti-GFAP Polyclonal Antibody,...
Immunofluorescence (IF) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 30-80) antibody (ABIN726200) Formalin-fixed and paraffin embedded: human placenta tissue labeled with Anti-GFAP Po...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 30-80) antibody (ABIN726200) Formalin-fixed and paraffin embedded: rat brain tissue Anti-GFAP Polyclonal Antibody,...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 30-80) antibody (ABIN726200) Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit An...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 30-80) antibody (ABIN726200) Image provided by One World Lab validation program. A549 cells probed with Rabbit Ant...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 30-80) antibody (ABIN726200) Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in ...
Western Blotting (WB) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 30-80) antibody (ABIN726200) U251 Cells lysates probed with GFAP Polyclonal Antibody, unconjugated at 1:300 overn...
Flow Cytometry (FACS) image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 30-80) antibody (ABIN726200) RSC96 cells probed with GFAP Polyclonal Antibody, Unconjugated at 1:100 for 30 minut...
 image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 30-80) antibody (ABIN726200) Astrocytes were stained with GFAP polyclonal antibody . Expression of GFAP expression...
 image for anti-Glial Fibrillary Acidic Protein (GFAP) (AA 30-80) antibody (ABIN726200) Formalin-fixed and paraffin embedded mouse spinal cord stained with GFAP Polyclonal A...
Product cited in: Hu, Kodithuwakku, Zhou, Li, Han, Fang, Liu, Li: "Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway." in: Molecules (Basel, Switzerland), Vol. 22, Issue 6, 2017 (PubMed). Method employed by authors: Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (Sample species: Mouse (Murine)).

Sweeney, Qi, Xu, Yang: "Activation of hypothalamic astrocytes suppresses feeding without altering emotional states." in: Glia, Vol. 64, Issue 12, pp. 2263-2273, 2016 (PubMed).

Yan, Li, Li, Lin, Yang: "Osthole Protects Bone Marrow-Derived Neural Stem Cells from Oxidative Damage through PI3K/Akt-1 Pathway." in: Neurochemical research, 2016 (PubMed). (Sample species: Mouse (Murine)).

Zhao, Sun, Miao, Ji, Zhao, Sun, Miao, Yoshii, Mizushima, Wang, Zhang: "The autophagy gene Wdr45/Wipi4 regulates learning and memory function and axonal homeostasis." in: Autophagy, Vol. 11, Issue 6, pp. 881-90, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: Brain

Mori, Matsubara, Matsubara, Uchikura, Hashimoto, Fujioka, Matsumoto: "Stromal Cell-Derived Factor-1? Plays a Crucial Role Based on Neuroprotective Role in Neonatal Brain Injury in Rats." in: International journal of molecular sciences, Vol. 16, Issue 8, pp. 18018-32, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Rat (Rattus)). Further details: Sample: Brain

Shan, Huang, You, Yuan, Gao, Hu, Du: "High efficiency intracellular transport of cationic peptide stearate for gene delivery in tumor cells and multipotent stem cells." in: Journal of biomedical nanotechnology, Vol. 10, Issue 11, pp. 3231-43, 2015 (PubMed).

Fan, Hu, Ji, Sun, Xiong, Yang, Liu: "Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons." in: In vitro cellular & developmental biology. Animal, Vol. 50, Issue 4, pp. 304-12, 2014 (PubMed). (Sample species: Human). Further details: Sample: bone marrow cells

Xiang, Wei, Chen, Liu, Liu, Wang, Zhang: "Anti-inflammatory effect of acetylpuerarin on eicosanoid signaling pathway in primary rat astrocytes." in: Journal of molecular neuroscience : MN, Vol. 52, Issue 4, pp. 577-85, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Sample: braistrocytes

Ma, Cao, Li, Gao, Qi, Zhao, Du, Xue, Peng, Wen, Chen, Ning, Huang, Zhang, Gao, Yu, Chen: "Dapper1 promotes autophagy by enhancing the Beclin1-Vps34-Atg14L complex formation." in: Cell research, Vol. 24, Issue 8, pp. 912-24, 2014 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)). Further details: Sample: Brain

Du, Liu, Chen, Dou, Lei, Chang, Cai, Cui, Yang, Sun, Li, Jiang: "Targeting the SMO oncogene by miR-326 inhibits glioma biological behaviors and stemness." in: Neuro-oncology, 2014 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Human).

Zuo, Wang, Li, Lin, Duan, Qi, Li, Sun, Wu: "Existence of glia mitigated ketamine-induced neurotoxicity in neuron-glia mixed cultures of neonatal rat cortex and the glia-mediated protective effect of 2-PMPA." in: Neurotoxicology, Vol. 44, pp. 218-30, 2014 (PubMed). (Sample species: Rat (Rattus)).

Hu, Zheng, Jia, Li, Dong, Wang, Lin, Zhang, Zhao, Xu, Zhou, Yuan, Zhang, Ren: "Neuroprotection effect of interleukin (IL)-17 secreted by reactive astrocytes is emerged from a high-level IL-17-containing environment during acute neuroinflammation." in: Clinical and experimental immunology, Vol. 175, Issue 2, pp. 268-84, 2014 (PubMed).

Zhao, Zhao, Wang, Xu, Miao, Feng, Chen, Kovács, Fan, Zhang: "Mice deficient in Epg5 exhibit selective neuronal vulnerability to degeneration." in: The Journal of cell biology, Vol. 200, Issue 6, pp. 731-41, 2013 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: brain

Liu, Xue, Tang, Hou, Qi, Chen, Chen, Zhang, Chen, Xu: "A simple method for isolating and culturing the rat brain microvascular endothelial cells." in: Microvascular research, Vol. 90, pp. 199-205, 2013 (PubMed). (Sample species: Rat (Rattus)).

Xiang, Chen, Liu, Liu, Wei, Sun, Wang, Zhang: "Inhibition of sPLA?-IIA prevents LPS-induced neuroinflammation by suppressing ERK1/2-cPLA?? pathway in mice cerebral cortex." in: PLoS ONE, Vol. 8, Issue 10, pp. e77909, 2013 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Rat (Rattus)).

Zhao, Zhao, Miao, Wang, Sun, Zhang: "The p53-induced gene Ei24 is an essential component of the basal autophagy pathway." in: The Journal of biological chemistry, Vol. 287, Issue 50, pp. 42053-63, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Sample: brain (cortex)

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