CCR2 antibody (Extracellular, N-Term)

Catalog No. ABIN7886264

The Rabbit Polyclonal anti-CCR2 antibody is suitable to detect CCR2 in samples from Mouse. It has been validated for WB, FACS, IHC and LCI.

Quick Overview for CCR2 antibody (Extracellular, N-Term) (ABIN7886264)

Target: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCR2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Product Details anti-CCR2 Antibody

Binding Specificity: AA 19-32, Extracellular, N-Term

Purpose: A Rabbit Polyclonal antibody to CCR2 (extracellular)

Sequence: (C)HSLFTRSIQE LDEG

Predicted Reactivity: Rat - 13 out of 14 amino acid residues identical Won't recognize CCR2 from human samples

Characteristics: Anti-CCR2 (extracellular) Antibody (ABIN7581842) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CCR2 from mouse and rat samples. The antibody will not work with human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HSLFTRSIQELDEG, corresponding to amino acid residues 19 - 32 of mouse CCR2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CCR2

Target: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Alternative Name: CCR2 

Background:

Synonyms: C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CD192

Description: CC chemokine receptor 2 (CCR2) is one of 19 members of the chemokine receptor subfamily of class A G-protein-coupled receptors (GPCRs). CCR2 is expressed in monocytes, immature dendritic cells, and T-cell subpopulations, and mediates acute inflammation by driving leukocyte migration to damaged or infected tissues towards chemokine ligands such as CCL2. Elevated expression of chemokines and their receptors such as CCR2 and its ligands can contribute to chronic inflammation and malignancy and hence these molecules are implicated in numerous inflammatory and neurodegenerative diseases including atherosclerosis, multiple sclerosis, asthma, neuropathic pain, and diabetic nephropathy, as well as cancer. These disease associations have motivated numerous studies in search of therapies that target the CCR2-chemokine axis1,2.As with most G-protein coupled receptors (GPCRs), chemokine receptors transmit signals across cell membranes by means of extracellular ligand and intracellular G-protein binding. CCR2 has seven transmembrane alpha helixes, and can take distinct conformational states that are necessary for chemokine/ligand binding, G-protein binding, activation, inactivation, and signal transmission3.CCR2 can form homo- or heterodimers with other chemokine receptors. Homodimerization may be necessary for CCR2 chemotactic activity and occurs in the absence of the ligand. CCR2/CCR5 heterocomplexes activate calcium response and support cell adhesion rather than chemotaxis, whereas CCR2/CCR4 heterodimers have an allosteric trans-inhibitory effect on CCL2 binding2.CCR2 and its main ligand CCL2 are abundantly expressed in both the central and peripheral nervous system and have well established roles in osteoarthritis pain4, cerebral ischemia5, and depressive disorders6, among others.

Gene ID: 12772

UniProt: P51683

Pathways: cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process